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Home / Proteins & Peptide

Recombinant Human G-CSF (CSF3)

SKU : BHP10900472

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Z102755
Description	G-CSF is secreted by monocytes, macrophages, and neutrophils after cell activation. It is also produced by stromal cells, fibroblasts, endothelial cells, epithelial carcinomas, acute myeloid leukemia cells, and various tumor cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL1 and GM-CSF. Comparison of the primary sequence of G-CSF with those of GM-CSF and M-CSF, shows that the three factors are not related to each other. Murine and human G-CSF share a sequence homology (protein) of approximately 70% and exhibit cross reactivity. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophils and granulocytes lineage in a dose-dependent manner. Expressed in human 293 cells, it is a monomeric glycoprotein with a molecular mass of 21 to 25 kDa.
Alternative Names	CSF-3 ; MGI-1G ; Pluripoietin ; Human Granulocyte Colony Stimulating Factor (CSF3) ; GCSF
Quantity	10 µg
Regulatory	RUO
Source	HEK293
Host	Human
Endotoxin Level	<1 EU/µg of recombinant protein as determined by the LAL method
Biological Activity Comment	The EC(50) as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line) was found to be ≤ 0.1 ng/mL.

Weight	21 to 25 kDa, monomer,

Description	A quick spin of the vial followed by reconstitution in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Format	Lyophilized Powder 1x PBS
Purity	>95% as determined by SDS-PAGE
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.

Immunogen Species	Homo Sapiens (Human)

MSDS	Growth Factors, Cytokines & Kinases MSDS
Other	Growth Factor and Cytokine Brochure

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SKU	Supplier No.	Size	Your price	Quantity
BHP10900472	Z102755	10µg	$264.44		Add to Cart

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