Fractalkine is a type I membrane protein that signals through the CX3CR1 receptor. The extracellular domain of Fractalkine is released by protease cleavage – producing soluble Fractalkine. Fractalkine has been found in many tissues and shown to be up-regulated in endothelial cells and microglia in response to inflammatory signals. Fractalkine is also involved in leukocyte adhesion as well as attracting monocytes, microglia cells and NK cells.
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein
Molecular Weight
8.9 kDa
Quantity
1.0 mg
Source
E. coli
Usage
For research use only. Not for diagnostic or therapeutic use.
Accession Number
P78423
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Bioactivity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED(50) for this effect is typically 20100 ng/mL,
Gel Image
Gene Id
6376
Gene Name
CX3CL1
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.Avoid repeated freeze/thaw cycles.