Expressed in the pituitary, ovaries, and in some other tissues, FST is a high affinity activin binding protein that can act as an activin antagonist. FST was originally discovered through its suppressive effect of the expression and secretion of the pituitary follicle stimulating hormone. Besides activin, FS has also been shown to bind with multiple BMPs, Myostatin, GDF-11, and TGF-beta-1. Human Follistatin contains three cysteine-rich domains (called Follistatin-like domains), 3 Kazal-like domains and one TGF-beta binding domain.
Alternative Names
FS ; Activin-binding protein
Quantity
10 µg
Regulatory
RUO
Source
E. coli
Host
Human
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Biological Activity Comment
The ED(50) was determined by the ability to neutralize Activin A inhibitory effect of mouse MPC-11 cells and was determined to be 0.1-0.4 µg/mL in the presence of 7.5 ng/mL Activin A.
Weight
31.0 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderRecombinant Human Follistatin was lyophilized at 1.0 mg/mL containing no additives.
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.