Flt3-Ligand Human Recombinant produced in HEK293 cells is a glycosylated, polypeptide chain, migrates as a diffuse band on SDS-PAGE due to heterogeneous glycosylation at 30kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.
Regulatory
RUO
Source
HEK293 (Human Embryonic Kidney cell line)
Biological Activity Comment
The ED50 as determined by the dose dependent proliferation of human OCI-AML5 cells is 0.5 to 1.0 ng/ml (Specific Activity: 2.0 x 1,000,000 – 1.0 x 1,000,000 units/mg).
Nature
Recombinant
Uniprot
P49771
Description
Formulation: The protein was lyophilized with no additives. Solubility: It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Format
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. ; ; ; (b) Analysis by SDS-PAGE.