FGF-8 is a heparin binding protein of the FGF family which works to control cell division, differentiation, and function. These proteins are especially prominent during prenatal development and postnatal growth. FGF-8 can be spliced in four different ways. The products of the FGF-8 splicing events are as follows: FGF8A, 8B, 8E and 8F. This recombinant product represents isoform 8B and is expressed in human 293 cells as a monomeric glycoprotein with a molecular mass of 30 to 45 kDa. This wide molecular mass is due to glycosylation, which is absent when this cytokine is expressed in E. coli.
<1 EU/µg of recombinant protein as determined by the LAL method
Biological Activity Comment
The EC(50) as determined by dose-dependent stimulation of the proliferation of the Balb/3T3 cell line was found to be ≤ 60 ng/mL.
Weight
30 to 45 kDa, monomer, glycosylated
Description
A quick spin of the vial followed by reconstitution in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Format
Lyophilized Powder10 mM Tris-HCl pH 7.4 + 800 mM NaCl
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.