A member of the FGF family, FGF4 plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF4 binds to anionic glycosaminoglycans heparin and heparin sulfate with high affinity and mediates its diverse functions through the FGF receptors (FGFR) 1c, 2c, 3c, and 4. Human FGF4 is known to exhibit cross species activity with mouse, rat, bovine and canine FGF4. Recombinant Human FGF4 expressed in human 293 cells is a monomeric glycoprotein with a molecular mass of 17 and 27 kDa.
<1 EU/µg of recombinant protein as determined by the LAL method
Biological Activity Comment
The EC(50) as determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line was found to be ≤1.25 ng/mL.
Weight
17 and 27 kDa, monomer, glycosylated
Description
A quick spin of the vial followed by reconstitution in sterile 1xPBS.
Format
Lyophilized Powder1x PBS
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.