A member of the FGF family, FGF4 plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF4 binds to anionic glycosaminoglycans heparin and heparin sulfate with high affinity and mediates its diverse functions through the FGF receptors (FGFR) 1c, 2c, 3c, and 4. Human FGF4 is known to exhibit cross species activity with mouse, rat, bovine and canine FGF4. Recombinant Human FGF4 is a single polypeptide of 20 kDa.
<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment
The ED50 as determined by the dose-dependent stimulation of mouse fibroblasts was <0.2 ng/ml
Weight
20.0 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderLyophilized from a 0.2 μm filtered solution in PBS (pH 8.0)
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.