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Recombinant Human FGF2

SKU : BHP10900283

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Z101455
Description	Basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.
Alternative Names	Fibroblast growth factor 2 ; FGF-2 ; Heparin-binding growth factor 2 ; HBGF-2 ; bFGF
Quantity	50 µg
Regulatory	RUO
Source	E. coli
Host	Human
Endotoxin Level	<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment	The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.

Weight	17.0 kDa

Description	A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL is recommended. Please note, filter sterilization is a must following reconstitution. This solution can then be diluted into other buffers.
Format	Lyophilized Powder Lyophilized from a 0.2 μm filtered solution in PBS
Purity	>95% as determined by SDS-PAGE
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Immunogen Species	Homo Sapiens (Human)

Reference	Su, RJ et al. "Generation of iPS Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors." Methods Mol Biol :57-69 (2016). DOI: 10.1007/7651_2014_139. PubMed: 25403468. Application: Cell Culture.

MSDS	Growth Factors, Cytokines & Kinases MSDS
Other	Growth Factor and Cytokine Brochure

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SKU	Supplier No.	Size	Your price	Quantity
BHP10900283	Z101455	50µg	$64.20		Add to Cart

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