Human FGF19 is expressed during brain development and embryogenesis and is a high-affinity, heparin-dependent ligand for FGF receptor 4. Among FGF family members, human FGF19 is most closely related to murine FGF15 and chicken FGF19. Recombinant Human FGF19 is a non-glycosylated, 21.9 kDa protein that contains 195 amino acid residues.
Alternative Names
Fibroblast Growth Factor-19 ; FGFJ
Quantity
25 µg
Regulatory
RUO
Source
E. coli
Host
Human
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment
The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml
Weight
21.9 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderLyophilized from a 0.2 μm filtered solution in PBS
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.