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Home / Proteins & Peptide

Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155)(Q65I,C96S,N111G)

SKU : BHP10304460

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSH033502
Description	Recombinant Human FGF-2(Q65I;C96S;N111G) is produced by our E.coli expression system and the target gene encoding Met1-Ser155 is expressed.
Alternative Names	Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB
Reconstitution	Please refer to the printed manual for detailed information.
Background	Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.
Reactivity	Human
Formulation	Lyophilized from a 0.2 um filtered solution of 20mM Tris;200mM NaCl; pH7.5.
Host	E.coli
Accession Number	P09038-2
Endotoxin Level	< 1.0 EU per ?g as determined by the LAL method.
Mol Weight	17.2 kDa
Mw(kda)	17.2 kDa

Sequence Info	Met1-Ser155

Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	> 95% as determined by reducing SDS-PAGE.

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10304460	PKSH033502	10ug	$86		Add to Cart
BHP10304460	PKSH033502	50ug	-		Add to Cart

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