Recombinant Human F-box/WD repeat-containing protein 7(FBXW7)

Mfr.No.	CSB-CF822163HU
Alternative Names	Archipelago homolog1 Publication ; hAgo1 Publication ; F-box and WD-40 domain-containing protein 7Curated ; F-box protein FBX301 Publication ; SEL-101 Publication ; hCdc4
Antigen	FBXW7
Tag	N-terminal 6xHis-tagged
Regulatory	RUO
Relevance	Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463).
Source	in vitro E.coli expression system
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Protein Name	Archipelago homolog1 Publication; Short name: hAgo1 Publication; F-box and WD-40 domain-containing protein 7Curated; F-box protein FBX301 Publication; SEL-101 Publication; hCdc4
Expression Region	1-707aa
Mol Weight	83.7kDa
Nature	recombinant
Protein Description	Full Length

Subcellular Location	Isoform 1: Nucleus, nucleoplasm, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm, SUBCELLULAR LOCATION: Isoform 3: Nucleus, nucleolus
Uniprot	Q969H0, Q969H0
Database Links	HGNC:16712 OMIM:606278 KEGG:hsa:55294 STRING:9606.ENSP00000281708 UniGene:Hs.561245
Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination
Pathway	Ubiquitin mediated proteolysis
Research Area	Cell Biology
Tissue Specificity	Isoform 1 is widely expressed. Isoform 3 is expressed in brain.
Weight	82.92kDa

Stotage Note	Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Buffer	Tris-based buffer，50% glycerol
Purity	Greater than 90% as determined by SDS-PAGE.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Storage Buffer	Tris-based buffer，50% glycerol