Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. The factor is being referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophils in vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils.
<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment
Activity was determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between 50-100 ng/mL.
Weight
8.5 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderLyophilized from as 0.2 μm filtered solution in PBS (pH 7.5)
Purity
>95% as determined by SDS-PAGE
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.