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Home / Proteins & Peptide

Recombinant Human ENA-78(5-78a.a.) ( rHuENA-78(CXCL5))

SKU : BHP11300414

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Mfr.No.	PR1014
Antigen	ENA-78(5-78a.a.) )
Description	Epithelial cell-derived neutrophil-activating peptide 78 (ENA-78) is a member of the CXC subfamily of chemokines that has the Glu-Leu-Arg (ELR) motif preceding the CXC motif. Similar to other ELR containing CXC chemokines, ENA-78 is a potent neutrophil chemoattractant and activator. Proteolysis of ENA-78 with cathepsin G and chymotrypsin have yielded N-terminally truncated variants with increased biological activities. ENA-70 and ENA-74 represent truncated recombinant ENA-78 variants missing 8 and 4 aa residues, respectively, from the N-terminus. Recombinant ENA-70 and ENA-74 have been shown to have increased potency in neutrophil chemotaxis and myeloperoxidase and elastase release assays.
Regulatory	RUO
Source	Escherichia coli.
Host	Human
Endotoxin Level	Less than 1EU/mg of rHuENA-78/CXCL5 as determined by LAL method.
Biological Activity Comment	Fully biologically active when compared to standard. Determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 5.0-10.0 ng/ml, corresponding to a Specific Activity of ＞1 x 105 IU/mg.
Nature	Recombinant

Weight	8.0 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.

Description	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Format	Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 50mM NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	>97% by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11300414	PR1014	5μg	$205.56		Add to Cart
BHP11300414	PR1014	20μg	$383.33		Add to Cart

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