Cystatin C has been a simple, accurate, and rapid endogenous biomarker of glomerular filtration rate (GFR) in research and clinical practice for testing kidney function. Its role has been studied in predicting newonset or deteriorating cardiovascular diseasea cysteine proteinase inhibitor that is involved in kidney function, cardiovascular disease and neurologic disorders. It has been shown to colocalize with amyloid beta in brain disorders such as Alzheimer disease. Cystatin C is expressed in human 293 cells as a monomer with a molecular mass of 12 to 13 kDa.
<1 EU/µg of recombinant protein as determined by the LAL method
Biological Activity Comment
The IC(50) as determined by the inhibition of Cystatin C on papin’s protease activity, measured by a colorimetric assay using L-BAPA as substrate, was found to be at 5 to 20 µg/mL
Weight
12 to 13 kDa, monomer, nonglycosylated
Description
A quick spin of the vial followed by reconstitution in sterile 1xPBS.
Format
Lyophilized Powder1x PBS
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.