CCL25 is constitutively expressed in epithelium and mucosal vessels in the small bowel, where it interacts with gut-homing B and T cells expressing its receptor, CCR9.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Formulation
Recombinant CCL25 was lyophilized from PBS pH 7.4.
Molecular Weight
14.0 kDa
Quantity
1.0 mg
Source
E. coli
Usage
For research use only. Not for diagnostic or therapeutic use.
Accession Number
O15444
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Bioactivity
Activity was determined by the ability to induce chemotaxis of purified human monocytes at concentrations ranging between 2-10 ng/mL.
Gene Id
6370
Gene Name
CCL25
Purity
>95% as determined by SDS-PAGE
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.Avoid repeated freeze/thaw cycles.