Recombinant full-length human BMX was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The BMX gene encodes a non-receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells. The BMX gene is located on chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked agammaglobulinemia. BMX is found to induce activation of the Stat signalling pathway.
Alternative Names
Bone marrow tyrosine kinase ; ETK ; PSCTK2 ; PSCTK3
Quantity
10 µg
Regulatory
RUO
Source
Insect Cells
Host
Human
Biological Activity Comment
The specific activity of BMX was determined to be 34 nmol/min/ml.
Weight
110.0 kDa
Format
LiquidRecombinant BMX is stored in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% Glycerol at 0.1 µg/µl.
Purity
>80% as determined by SDS-PAGE
Storage
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.