BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. As a
Alternative Names
BMP-2A ; Recombinant Human Bone Morphogenetic Protein 2 (BMP2) ; BMP-2
Quantity
10 µg
Regulatory
RUO
Source
HEK293
Host
Human
Endotoxin Level
<1 EU/µg of recombinant protein as determined by the LAL method
Biological Activity Comment
The EC(50) as determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) was found to be ≤ 60 ng/mL
Weight
28 kDa, disulfide linked homodimeric
Description
A quick spin of the vial followed by reconstitution in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Format
Lyophilized Powder2x PBS + 6% Ethanol
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.