The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice.
<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment
The ED50 was determined by the dose-dependent proliferation of Raji cells and was found to be <0.5ng/mL.
Weight
9.0 kDa
Description
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format
Lyophilized PowderLyophilized from a 0.2 μm filtered solution PBS
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.