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Home / Proteins & Peptide

Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein (Active)

SKU : BHP10302705

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSH030312
Description	A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331); constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272); constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus; constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Alternative Names	AMPK;AMPKa1
Reconstitution	Please refer to the printed manual for detailed information.
Reactivity	Human
Formulation	Supplied as sterile 50mM Tris; 200mM NaCl; 1mM EDTA; 1mM DTT; 0.5mM PMSF; 10% gly; 1mM GSH; pH 7.4
Host	Baculovirus-Insect Cells
Accession Number	P54619
Endotoxin Level	< 1.0 EU per ug of the protein as determined by the LAL method.
Bioactivity	The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Mol Weight	158 (38+30+90) kDa
Mw(kda)	158 (38+30+90) kDa

Sequence Info	Met 1-Pro 331

Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stotage Note	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Purity	> 90 % as determined by reducing SDS-PAGE.

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10302705	PKSH030312	50ug	$1191		Add to Cart

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