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Home / Proteins & Peptide

Recombinant GM-CSF (Sargramostim), Human (P. pastoris-expressed)

SKU : BHP11300298

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Mfr.No.	BK0341‐10μg
Alternative Names	Granulocyte Macrophage Colony Stimulating Factor ; CSF-2 ; MGI-1GM ; GM-CSF ; Pluripoietin-alpha ; Molgramostin ; Sargramostim
Antigen	GM-CSF (Sargramostim)
Description	Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds[2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities[4]. Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells[5].Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in P. pastoris a glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 26-32kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Regulatory	RUO
Source	P. pastoris
Host	Human
Endotoxin Level	<1.0EU/μg, determined by LAL method.
Biological Activity Comment	ED50< 0.2ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of > 5x 10ˆ6 units/mg.
Nature	Recombinant

Weight	26-32kDa, observed by SDS-PAGE.

Description	Reconstituted in ddH2O at 100 μg/ml.
Format	Lyophilized after extensive dialysis against 10 mM PB, pH7.0. Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95 % as analyzed by SDS-PAGE.
Storage	Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11300298	BK0341‐10μg	10μg	$188.67		Add to Cart
BHP11300298	BK0341‐10μg	1mg	$3086.67		Add to Cart
BHP11300298	BK0341‐10μg	50μg	$485.20		Add to Cart

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