Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph)

Mfr.No.	CSB-YP365448ENL
Alternative Names	APH(4)Hygromycin B phosphotransferaseHygromycin-B kinase
Tag	N-terminal 6xHis-tagged
Regulatory	RUO
Relevance	The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as dethyl analogs and destomycin.
Source	Yeast
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Protein Name	APH(4)Hygromycin B phosphotransferaseHygromycin-B kinase
Expression Region	1-341aa
Mol Weight	40.0 kDa
Nature	recombinant
Protein Description	Full Length

Uniprot	P00557, P00557
Database Links	KEGG:ag:CAA24743
Function	The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin.
Protein Families	Aminoglycoside phosphotransferase family
Research Area	Others
Weight	40kDa

Stotage Note	Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Buffer	Tris-based buffer，50% glycerol
Purity	Greater than 90% as determined by SDS-PAGE.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Storage Buffer	Tris-based buffer,50% glycerol