GenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags.
Alternative Names
Enteropeptidase ; ENTK ; PRSS7
Regulatory
RUO
Source
P. pastoris
Host
Porcine
Biological Activity Comment
One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.
Nature
Recombinant
Weight
Theoretical MW: 21,880 DaThe apparent MW on SDS-PAGE: about 40,000 Da
Description
Reconstituted in ddH2O or PBS at 100 μg/ml.
Format
GenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity. Sterile Filtered White lyophilized (freeze-dried) powder.
Storage
Store at -20°C after delivery.
Reviews of Recombinant Enterokinase, Light Chain, Porcine