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Home / Proteins & Peptide

Recombinant 2019-nCoV Spike Protein, Biotinylated (RBD-SD1, Avi-His Tag)

SKU : BHP10306059

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSR030478
Description	Biotinylated 2019-nCoV Spike Protein RBD-SD1 is produced by our Mammalian expression system and the target gene encoding Arg319-Ser591 is expressed with a Avi, 6His tag at the C-terminus.
Alternative Names	2019-nCov RBD Protein; 2019-nCoV S RBD Protein
Reconstitution	Please refer to the printed manual for detailed information.
Background	Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Reactivity	Virus
Tag	C-Avi-6His
Formulation	Supplied as a 0.2 um filtered solution of PBS, pH 7.4.
Host	Human Cells
Accession Number	QHD43416.1
Endotoxin Level	< 1.0 EU per ug as determined by the LAL method.
Bioactivity	Immobilized Human ACE-2-Fc(Cat#PKSR030492) at 2ug/ml (100 ul/well) can bind Recombinant 2019-nCoV Spike Protein, Biotinylated (RBD-SD1, Avi-His Tag) (Cat#PKSR030478). The ED50 of Recombinant 2019-nCoV Spike Protein, Biotinylated (RBD-SD1, Avi-His Tag) (Cat#PKSR030478) is 0.13 ug/ml.
Mol Weight	33.2 kDa
Mw(kda)	33.2 kDa

Sequence Info	Arg319-Ser591

Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stotage Note	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Purity	> 95 % as determined by reducing SDS-PAGE.

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10306059	PKSR030478	20ug	$390		Add to Cart
BHP10306059	PKSR030478	100ug	$980		Add to Cart

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