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Home / Proteins & Peptide

RBD-c-RAF, GST-tag

SKU : BHP18201184

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Mfr.No.	100519-1
Description	Human Ras Binding Domain (RBD) of Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1) also known as C-Raf and NS5 with N-terminal GST-tag, GenBank Accession No. NM_002880, a.a. 50-140, expressed in an E. coli expression system. MW=36 kDa.
Immunogen Region	50-140
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.
Species	Human
Application	Useful for studying RAS/RAF binding study as well as inhibitor screening for RAS/RAF interaction.
Notes	GppNHp (or GDP) loaded His-tag KRAS(G12C) at various concentrations (Biohippo #100641 and Biohippo #100640) was incubated with 2.5 nM GST-tag RBD-RAF1 for 1 hour at room temperature. KRAS(G12C)/RBD-RAF1 binding was analyzed by AlphaScreening using GSH-Acceptor beads and Nickle Donor beads (PerkinElmer). Avoid freeze/thaw cycles.
GenBank	NM_002880
Mw(kda)	36 kDa

Synonyms	C-Raf, NS5
Uniprot	P04049

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -70°C.

Reference	1. Rooij, J., et al., Oncogene. 1997;14:623-625. 2. Bhaswati, P., et al., Nature Genetics. 2007;39:1007-1012.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201184	100519-1	100 ug	$385		Add to Cart

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