Human recombinant RB protein (773-end) with N-terminal GST-tag (GenBank Accession No. NM_000321), MW=43.5 kDa, expressed in an E. coli expression system.
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species
Human
Application
Useful as a substrate for kinase assays, protein binding studies, Western blot standard
Notes
Avoid freeze/thaw cycles.
GenBank
NM_000321
Mw(kda)
43.5 kDa
Synonyms
Retinoblastoma protein
Uniprot
P06400
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Hanahan, D. et al., Cell 100, 57-70 (2000). 2. Dahiya, A. et al., Molec. Cell 8: 557-568 (2001).