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Home / Proteins & Peptide

Rat PDE7B, GST-tag

SKU : BHP18200527

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BPS Bioscience

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Mfr.No.	60075
Immunogen Region	108-446(end)
Tag	N-terminal GST-tag
Description	Rat PDE7B (GenBank Accession No. NM_080894), a.a. 108-446(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=324 pmol/min/ug. Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Formulation	45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 18 mMglutathione, 10% glycerol, and 3 mM DTT.
Species	Rat
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE7B kit, Cat. # 60371. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 0.1 mg/ml BSA, 200 uM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of rPDE7B at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_080894
Mw(kda)	65 kDa

Synonyms	PDE7B, phosphodiesterase 7B
Uniprot	Q8VIE4

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Gardner, C., et al. (2000). Biochem Biophys Res Commun. May 27;272(1):186-92. 2. Reyes-Irisarri, E., et al. (2005). Neuro-science 132(4):1173-85. 3. Zhang, L., et al. (2008). Proc. Natl. Acad. Sci. USA Dec 9;105(49):19532-7.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200527	60075	10 ug	$500.50		Add to Cart

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