Rat PDE10A GenBank Accession No. NM_022236), a.a. 449-end with N-terminal GST-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9
Specific activity: >= 8339 pmol/min/ug
Unit definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3' , 5' -cAMP to5' -AMP per minute at 37°C.
Immunogen Region
449-end
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species
Rat
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 200 uM cAMP, and 2.5 kU 5 - nucleotidase, 37°C, 20 min. Quantified by 5'-nucleotidasecleaving the 5'-AMP product and releasing the phosphate group which is detected byMalachite Green Reagent.
Avoid freeze/thaw cycles.
GenBank
NM_022236
Mw(kda)
66 kDa
Synonyms
PDE10, phosphodiesterase 10A
Uniprot
Q9QYJ6
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Siuciak, J.A. et al., Neuropharmacology 51 (2), 374-385 (2006). 2. Hu,H. et al., Eur. J. Neurosci. 20 (12), 3351-3363 (2004).