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Home / Proteins & Peptide

Rat ITK, GST-tag

SKU : BHP18200245

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BPS Bioscience

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Mfr.No.	40447
Description	Rat recombinant IL2-inducible T-cell kinase (ITK), also known as Lyk and TSK (GenBank Accession #NM_001108825), a.a. 352-620(end) with N-terminal GST-tag, MW= 57 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 1.0 pmol/min/ug
Immunogen Region	352-620(end)
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
Species	Rat
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Z-Lyte (Invitrogen) screening protocol and condition were used. Enzyme reaction was conducted at 30°C for 1 hours with 2 uM Tyr 01 Peptide in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl₂, 1 mM EGTA. After the 1 hour incubation, diluted Development Reagent B was added. Avoid freeze/thaw cycles.
GenBank	NM_001108825
Mw(kda)	57 kDa

Synonyms	ITK, IL2-inducible T-cell kinase , Lyk, TSK
Uniprot	D4A7W7

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Hawkins, J. and Marcy, A. (2001). Protein Expr. Purif. 22 (2): 211-9. 2. Kosaka,Y., et al. (2006). Trends Immunol. 27 (10): 453-60. 3. Tanaka, N., et al. (1993). FEBS Lett. 324 (1): 1-5.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200245	40447	10 ug	$390.50		Add to Cart

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