Human Receptor Activator of Nuclear Factor kappa B Ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 and TRANCE, (Genbank Accession No. NM_003701), a.a. 64-245, with an N-terminal His-tag, expressed in a HEK293 cell expression system. MW = 23 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
64-245
Tag
N-terminal His-Tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Notes
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
NM_003701
Mw(kda)
23 kDa
Synonyms
Receptor Activator of Nuclear Factor kappa B, TRANCE ligand, TNFRSF11A, Human Receptor Activator of Nuclear Factor kappa B Ligand
Uniprot
O14788
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Yasuda, H., J. American Chemical Society., 1998 March; 126(4): 3597-3602.2. Takayanagi, H., J. Developmental Cell. 20011 May; 20(5): 2.