Human Receptor Activator of Nuclear FactorkappaB (RANK), also known as TRANCE Receptor and TNFRSF11A, (Genbank Accession No. NM_003839), a.a. 30-212, fused at the C-terminus to the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTag technology. MW = 49 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Human
Application
Useful for studying protein binding and for screening small molecules and antibodies.
Notes
This protein runs at a higher MW by SDS-PAGE due to glycosylation. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_003839
Mw(kda)
49 kDa+glycans
Synonyms
Receptor Activator of Nuclear Factor kappaB, TRANCE Receptor, TNFRSF11A
Uniprot
Q9Y6Q6
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Annis, A., J. American Chemical Society., 2004 May; 126(4): 15495-15503.2. Yan, T., J. Cellular Biochemistry. 2001 Aug ; 83(2):320-325.