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Home / Proteins & Peptide

PYK2, His-tag

SKU : BHP18200253

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BPS Bioscience

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Mfr.No.	40480
Description	Human PYK2 (GenBank Accession No. U33284), full length with N-terminal His-tag, MW=120 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 5.6 pmol/min/ug.
Immunogen Region	full length
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	ADP-Glo assay with 0.1 mg/ml poly-(Glu4:Tyr) as a substrate and 100 mM ATP at final concentration. Reaction was done for 60 minutes at 30°C. ADP-Glo reagents were added for 45 minutes at RT, and Kinase detection reagents for 55 minutes at RT, followed by luminescence detection. Avoid freeze/thaw cycles.
GenBank	U33284
Mw(kda)	120 kDa

Synonyms	CADTK, CAK beta, CAK-beta, CAKB, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, PKB, Proline-rich tyrosine kinase 2, protein kinase B, protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK, Re
Uniprot	Q14289

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Cain R.J. et al., J Cell Biol. 2010 Mar 22,188(6):863-76. 2. Collins M. et al., Mol Immunol. 2010 May,47(9):1665-74.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200253	40480	10 ug	$390.50		Add to Cart

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