Human Prolyl oligopeptidase (POP)/Prolyl endopeptidase (PREP) (GenBank Accession No. NM_002726) full length with N-terminal His-tag, MW = 81.6 kDa, expressed in an E. coli expression system.
Specific activity: >= 12 pmol/min/ug.
Background
Prolyl oligopeptidase (POP), also known as prolyl endopeptidase(PREP), is a cytosolic serine peptidase involved in the maturation and degradation ofpeptide hormones and neuropeptides.
Immunogen Region
full length
Tag
N-terminal His-tag
Formulation
45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 225 mMimidazole, 10% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mMTris (pH 7.5), 0.1% BSA, 20 uM substrate(A-P-AMC), and 5 ng/ul POP at roomtemperature for 30 min.