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Home / Proteins & Peptide

PRMT8, His-FLAG-tags

SKU : BHP18200429

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Mfr.No.	51052
Description	Human PRMT8 (protein arginine methyltransferase 8), GenBank Accession No. NM_019854, a.a. 61-394(end) with N-terminal His-FLAG-tag, MW=40.8 kDa, expressed in Sf9 cells using a Baculovirus expression system. host species: insect Host species: insect Host cell line: Sf9 Specific activity: 2.22 pmol/min/ug
Background	Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
Immunogen Region	61-394(end)
Tag	N-terminal His-FLAG-tags
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ug/ml FLAG peptide, 3 mM DTT, 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (20 mM phosphate buffer pH 7.4, 20 uM S-adenosyl methionine, and 1-5 ng methyltransferase PRMT8) added to the wells coated with the substrate. Incubate at RT for 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally,add HRP chemiluminescent substrates and read luminesence. Avoid freeze/thaw cycles.
GenBank	NM_019854
Mw(kda)	40.8 kDa

Synonyms	protein arginine N-methyltransferase 8, PRMT8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4
Uniprot	Q9NR22

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Bedford MT., et al. J Biol Chem. 2007 Dec 14;282(50):36444-53. 2. Taneda T., et al Brain Res. 2007 Jun 25;1155:1-9.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200429	51052	20 ug	$495		Add to Cart

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