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Home / Proteins & Peptide

PRMT6 (HRMT1L6), His-tag

SKU : BHP18200428

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Mfr.No.	51049
Description	Human PRMT6 (GenBank Accession No. NM_018137), a.a. 2-375(end), with N-terminal His-tag, MW=43 kDa, expressed in E. coli. Host cell line: E. coli Specific activity: 0.015 pmol/min/ug
Background	Arginine methyltransferase that can both catalyze the formation of monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 Arg-2 to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 Lys-4 (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 Arg-3 (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
Immunogen Region	2-375(end)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (20 mM phosphate buffer pH 7.4, 20 uM S-adenosylmethionine, and 1-5 ng methyltransferase PRMT6) is added to microwells coated with histone substrate. Incubate at 30°C for 2 hr. Add antibody against methylated Arg residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence. Avoid freeze/thaw cycles.
GenBank	NM_018137
Mw(kda)	43 kDa

Synonyms	PRMT6, HRMT1L6, protein arginine N-methyltransferase 6
Uniprot	Q96LA8

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Hyllus D. et al., J. Genes Dev. 21 (24), 3369-3380 (2007). 2. Guccione E. et al., Nature 449 (7164), 933-937 (2007).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200428	51049	20 ug	$363		Add to Cart

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