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Home / Proteins & Peptide

PRMT5/MEP50, FLAG-tag, His-tag (HEK293-derived)

SKU : BHP18200425

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BPS Bioscience

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Mfr.No.	51045
Description	Complex of human PRMT5 (GenBank Accession No. NM_006109), a.a. 2-end, with N-terminal FLAG-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), a.a. 2-end, with N-terminal His tag, MW=37.5 kDa, expressed in FreeStyle 293-F cells. host species: human Host species: human Host cell line: FreeStyle 293-F cells
Background	Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
Immunogen Region	2-end (both)
Tag	N-terminal FLAG-tag (PRMT5), N-terminal His-tag (MEP50)
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 ug/ml Flag peptide, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (20 mM phosphate buffer pH 7.4, 20 uM S-adenosyl methionine, and 10-100 ng methyltransferase PRMT5) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence. Avoid freeze/thaw cycles.
GenBank	NM_006109 (PRMT5), NM_024102 (MEP50)
Mw(kda)	73 kDa (PRMT5), 37.5 kDa (MEP50)

Synonyms	PRMT5, HRMT1L5, IBP72, JBP1, SKB1,
Uniprot	O14744

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Zhao Q. et al., J. Nat. Struct. Mol. Biol. 16 (3), 304-311 (2009). 2. Wang L et al., Mol. Cell. Biol. 28 (20), 6262-6277 (2008). Application Reference(s): Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and Its Substrate RNA Splicing Factor SF3B2 (2015)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200425	51045	20 ug	$654.50		Add to Cart

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