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Home / Proteins & Peptide

PRMT5/MEP50, Biotin-Labeled

SKU : BHP18200473

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BPS Bioscience

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Mfr.No.	52102
Description	PRMT5/MEP50 complex of human PRMT5 (GenBank Accession No. NM_006109), amino-acids 2-637(end), with N-terminal FLAG-Avi tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102) with N-terminal His-Tag, amino-acids 2-342(end), MW=38 kDa, coexpressed in HEK293 cells and enzymatically biotin-labeled using Avi-tag technology. host species: mammalian (human) Host species: mammalian (human) Host cell line: HEK293
Background	Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
Immunogen Region	2-end (both)
Tag	N-terminal FLAG-Avi-tag (PRMT5), N-terminal His-tag (MEP50)
Formulation	40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ug/mL FLAG peptide, 20% glycerol and 3 mM DTT.
Species	Human
Notes	Avoid freeze/thaw cycles.
GenBank	NM_006109 (PRMT5), NM_024102 (MEP50)
Mw(kda)	73 kDa (PRMT5), 38 kDa (MEP50)

Synonyms	PRMT5, HRMT1L5, IBP72, JBP1, SKB1, PRMT Protein, MEP50
Uniprot	PRMT5: O14744; MEP50: Q9BQA1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Reference	1. Zhao Q. et al., J. Nat. Struct. Mol. Biol. 16 (3), 304-311 (2009). 2. Wang L et al., Mol. Cell. Biol. 28 (20), 6262-6277 (2008).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200473	52102	10 ug	$566.50		Add to Cart

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