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Home / Proteins & Peptide

PRMT3, GST-tag

SKU : BHP18200424

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Mfr.No.	51043
Description	Human PRMT3 (GenBank Accession No. NM_005788), a.a. 2-end, with N-terminal GST-tag, MW=86 kDa, expressed in an E. coli expression system. Host cell line: E. coli Specific activity: 490 pmol/min/ug
Background	PRMT3 is a protein arginine methyltransferase that catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins. 40S ribosomal protein S2 is a physiological substrate of PRMT3 and PRMT3 regulates ribosome biosynthesis at a stage beyond pre-rRNA processing. DAL-1 (differentially expressed in adenocarcinoma of the lung)/4.1B is a tumor suppressor gene that can significantly inhibits PRMT3 methylation of cellular substrates. Modulation of post-translational methylation may be an important mechanism through which DAL-1/4.1B affects tumor cell growth.
Immunogen Region	2-end
Tag	N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (20 mM phosphate buffer pH 7.4, 20 uM S-adenosyl methionine, and 1-10 ng methyltransferase PRMT3) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence. Avoid freeze/thaw cycles.
GenBank	NM_005788
Mw(kda)	86 kDa

Synonyms	PRMT3, HRMT1L3
Uniprot	Q8WUV3

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Choi S. et al., Biochim Biophys Acta. 2008 Sep,1780, 9, :1062-9. 2. Bachand F. and Silver P.A., EMBO J. 2004 Jul 7,23, 13, :2641-50.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200424	51043	50 ug	$363		Add to Cart

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