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Home / Proteins & Peptide

PRMT1, GST-tag (E. coli-derived)

SKU : BHP18200422

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Mfr.No.	51040
Description	Human PRMT1 (GenBank Accession No. NM_001536), a.a. 2- end, with N-terminal GST-tag, MW= 68 kDa, expressed in an E. coli expression system. Host cell line: E. coli Specific activity: 0.52 pmol/min/ug
Background	PRMT1 is a protein arginine methyltransferase that functions as a histone methyltransferase specific for histone H4. PRMT1 specifically methylates arginine-3 of histone H4 in vitro and in vivo. Methylation of arg3 by PRMT1 facilitates subsequent acetylation of histone H4 tails by p300. PRMT1 performs methylation of arg31 of STAT1 and this is required for transcription induced by IFN-alpha/IFN-beta. Arginine methylation of STAT1 is an additional posttranslational modification regulating transcription factor function, and alteration of arginine methylation might be responsible for the lack of interferon responsiveness observed in many malignancies. Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze asymmetric dimethylarginine (ADMA), while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. PRMT1 is the primary methyltransferase that deposits the ADMA mark, and it accounts for over 90% of this type of methylation.
Immunogen Region	2-end
Tag	N-terminal GST-tag
Formulation	40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 20% glycerol, 3mM DTT
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (20 mM phosphate buffer pH 7.4, 20 uM S-adenosyl methionine, and 1-5 ng methyltransferase PRMT1) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence. Avoid freeze/thaw cycles.
GenBank	NM_001536
Mw(kda)	68 kDa

Synonyms	PRMT1, HRMT1L2, IR1B4, HCP1
Uniprot	Q99873

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Le Romancer,M. et al., Mol. Cell 31, 2, 212-221, 2008. 2. Fronz,K et al., J. Biol. Chem. 283, 29, 20408-20420, 2008.Application Reference(s): 1.A Sensitive Luminescent Assay for the Histone Methyltransferase NSD1 and Other SAM-Dependent Enzymes (2014) 2.Selective inhibitors of bacterial t-RNA-(N(1)G37) methyltransferase (TrmD) that demonstrate novel ordering of the lid domain (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200422	51040	50 ug	$363		Add to Cart

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