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Home / Proteins & Peptide

PRDM9, GST-Tag

SKU : BHP18200880

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BPS Bioscience

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Mfr.No.	100078
Description	Human PRDM9, also known as PR domain zinc finger protein 9, MEISETZ, and PR/SET domain 9, GenBank Accession No. NM_020227, a.a. 2-400, with an N-terminal GST-tag, expressed in an E. coli expression system. MW = 73 kDa.
Immunogen Region	2-400
Tag	N-terminal FLAG-Tag
Formulation	40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20mM glutathione, 3mM DTT and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	For standard, H3, 1-21 K4 (2Me)-Biotin was coated onto a neutravadin plate at varying concentrations. For test lots, H3,1-21-Biotin was coated onto a neutravadin plate at 0.4 ug/ml. Reaction mixture contained 50 mM Tris buffer pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20, 40 uM S-adenosylmethionine, and various amounts of PRDM9 incubated for 1 hour at room temperature. Followed by 1 hour incubation with primary antibody against H3 K4 (2Me), and 30 minute incubation with secondary antibody. Luminescence was developed with HRP substrate A and B (Pierce). Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank	NM_020227
Mw(kda)	73 kDa

Synonyms	PRDM9, PR domain zinc finger protein 9, MEISETZ, PR/SET domain 9
Uniprot	Q9NQV7

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution.
Storage	At least 6 months at -80°C.

Reference	1. Baudat, F., et al., Science. 2010 Feb; 327(5967): 836-40. 2. Parvanov, E., et al., Science. 2010 Feb; 317(5967): 835.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200880	100078	50 ug	$484		Add to Cart

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