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Home / Proteins & Peptide

PP2A-alpha, His-tag/PP2AR, GST-tag

SKU : BHP18200046

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BPS Bioscience

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Mfr.No.	30056
Description	Protein complex of humanprotein phosphatase 2A catalytic subunit (PP2A), also known as TPD3, RP-C,PP2Ac, and PPP2CA (GenBank AccessionNM_002715, a.a. 2-309 with C-terminal Histag)and human protein phosphatase 2Aregulatory subunit (PP2AR) (GenBankAccession NM_014225, a.a. 2-589 with NterminalGST-tag). Co-expressed in abaculovirus-infected Sf9 cell expressionsystem. MW = 36 kDa (PP2A) and 92 kDa(PP2AR). host species: Sf9 cells Host species: Sf9 cells Specific activity: >=100 pmol/min/ug
Immunogen Region	2-309 (PP2A-alpha); 2-589 (PP2AR)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 20% glyceroland 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics and for inhibitor screening.
Notes	Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 3 mM DTT, 10 mM pNPP and PP2A. Assay was done at 37°C for 40 min, and the reaction progress was measured at 405 nm. Specific activity was calculated based on pNPP= 18,000 M-1cm-1. Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
GenBank	NM_002715 (PP2A-alpha); NM_014225 (PP2AR)
Mw(kda)	36 kDa (PP2A-alpha); 92 kDa (PP2AR)

Synonyms	protein phosphatase 2A catalytic subunit alpha, TPD3, RP-C, PP2Ac,PPP2CA, protein phosphatase 2A regulatory subunit, PP2AR
Uniprot	P67775; P30153

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Van Hoof, C. et al., Genomics. 1995; 28:261-272. 2. Chao, Y. et al., Molec. Cell. 2006; 23:535-546.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200046	30056	20 ug	$440		Add to Cart

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