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Home / Proteins & Peptide

PLK3, His-tag

SKU : BHP18200143

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Mfr.No.	40035
Description	Human Plk3 (GenBank Accession No. NM_004073), (a.a. 58-339) with N-terminal His-tag, MW=36.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Source: Baculovirus infected Sf9 cells Purity:>=80% by SDS-PAGE. Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 uM Na orthovanadate, 0.5 mM ATP. host species: Sf9 cells Host species: Sf9 cells Specific activity: 350 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of substrate per minute at pH 7.5 and 30°C.
Background	PLK3 or Polo-like kinase 3 is a cytokine-inducible kinase that is a member of the Polo family of serine/threonine protein kinases. PLK3 contains both a catalytic domain and a putative regulatory domain and plays an important role in the regulation of cell cycle progression and tumorigenesis. PLK3 is closely associated with centrosomes in several human cell lines and its localization is dependent on the integrity of microtubules. Hence, PLK3 is also involved in regulating microtubule dynamics and centrosomal function.
Immunogen Region	58-339
Tag	N-terminal His-tag
Formulation	50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, 50% glycerol and 1 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200uM ATP, 0.01% Brij-35 and 2 uM substrate (SER/THR 16, Invitrogen) at room temperature for 1 hour. Avoid freeze/thaw cycles.
GenBank	NM_004073
Mw(kda)	36.7 kDa

Synonyms	CNK, FNK, PRK, polo-like kinase 3
Uniprot	Q9H4B4

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Li, Z., et al., J. Biol. Chem. 280 (17), 16843-16850 (2005). 2.Xie,S., et al., Oncogene 23 (21), 3822-3829 (2004).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200143	40035	10 ug	$390.50		Add to Cart

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