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Home / Proteins & Peptide

PLK1, His-tag

SKU : BHP18200141

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Mfr.No.	40033
Description	Human Plk1 (GenBank Accession No. NM_005030), full length with C-terminal His-tag, MW=68 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 125 pmole/min/ug Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of substrate per minute at pH 7.5 and 30°C.
Background	PLK1 is a member of the Polo-Like Kinase family that localize to centrosomes or spindle pole bodies and undergo dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics. PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death.
Immunogen Region	full length
Tag	C-terminal His-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 3 mM DTT and 10% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35 and 2 uM substrate (Ser/Thr Peptide 16, Invitrogen) at 30° for 1 hour. Avoid freeze/thaw cycles.
GenBank	NM_005030
Mw(kda)	68 kDa

Synonyms	STPK13, polo-like kinase 1
Uniprot	P53350

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Fabbro, M. et al., Dev. Cell 9 (4), 477-488. (2005). 2. Liu, X., et al., Mol. Cell. Biol. 25, 4993-5010 (2005).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200141	40033	10 ug	$390.50		Add to Cart

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