Human PLA2G7, (GenBank Accession No. NM_005084), a.a. 22-441(end), with C-terminal His-tag, MW=49.2 kDa, expressed in a mammalian cell expression system.
host species: mammalian (human)
Host species: mammalian (human)
Immunogen Region
22-441(end)
Tag
C-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted using liposomally incorporated Red/Green BODIPY PC-A2 (5 uM, Invitrogen) as a substrate in a reaction buffer (50 mM Tris, pH 8.8, 100 mM NaCl, 1 mM CaCl2). Reaction progress was monitored at ex480/em533 continuously at room temperature.
Avoid freeze/thaw cycles.
GenBank
NM_005084
Mw(kda)
49.2 kDa
Synonyms
Phospholipase A2 G7, Lp-PLA2, PAF-AH
Uniprot
Q13093
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Berg M., et al., Mol Cancer. 9:100. (2010). 2. Kopec AK., et al., Toxicol Appl Pharmacol.256(2):154-67. (2011).