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Home / Proteins & Peptide

PLA2G4A, FLAG-His-tags

SKU : BHP18200533

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BPS Bioscience

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Mfr.No.	60094
Description	Human PLA2G4A, GenBank Accession No. NM_024420, a.a. 1-749 (end), with C-terminal FLAG-His-tag, MW=87 kDa, expressed in a baculovirus expression system. Host cell line: Sf9 Specific activity: >=57 pmol/min/ug.
Immunogen Region	1-749 (end)
Tag	C-terminal FLAG-His-tags
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ug/ml FLAG peptide, and 20% glycerol.
Species	Human
Notes	PLA reaction was performed using the 1-Hexadecanoyl-2-(1-prenedecanoyl)-sn-Glycero-3-phosphocholine substrate. Enzyme reaction is conducted in 100 ul volume containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM CaCl₂, 0.5 mg/ml BSA, 10 uM substrate and 200 ng PLA2G4A. Reaction progress was monitored at ex345/em395 for 10 minutes continuously at room temperature. Avoid freeze/thaw cycles.
GenBank	NM_024420
Mw(kda)	87 kDa

Synonyms	Phospholipase A2 G4
Uniprot	P47712

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Chalimoniuk M. Postepy Biochem. 2012; 58(2):204-8. 2. Nadalin S, Giacometti J, et al., Prostaglandins Leukot Essent Fatty Acids. 2013; 88(2):185-90.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200533	60094	20 ug	$412.50		Add to Cart

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