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Home / Proteins & Peptide

PKM2 (PKM2/Variant 1), His-tag

SKU : BHP18200385

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Mfr.No.	50295
Description	Human Pyruvate kinase M2 (PKM2) also known as PKM1/M2, isoform a (GenBank Accession No. NM_002654) full length with N-terminal His-tag, MW = 58.9 kDa, expressed in an E. coli expression system. Specific activity: >=200,000 pmoles/min/ug.
Background	PKM1 and PKM2 are different splicing products of the PKM gene (exon 9 for PKM1 and exon 10 for PKM2). The M1 isoform (PKM1) is expressed in muscle and brain, and the M2 isoform is expressed during embryogenesis, in adipose tissue and pancreatic islets, and is the predominant form found in cancer cells.
Immunogen Region	full length
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 50 ul PKM2 reaction is conducted in abuffer containing 50 mM Tris (pH 7.4), 10mM MgCl₂, 50 mM KCl, 2 mM ADP, 10mM phosphoenolpyruvate (PEP) at roomtemperature for 15 min. ATP production isdetected using Kinase-Glo LuminescentKinase Assay Platform. Avoid freeze/thaw cycles.
GenBank	NM_002654
Mw(kda)	58.9 kDa

Synonyms	pyruvate kinase muscle, PKM2, cytosolic thyroid hormone-binding protein,CTHBP, thyroid hormone-binding protein 1, THBP1, opa-interacting protein 3,OIP3
Uniprot	P14618

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Chen, J., et al. 2011. Oncogene 30(42):4297-306. 2. Wong T.S. et al., Int. J. Cancer 123 (2), 251-257 (2008). 3. Christofk H.R. et al., Nature 452 (7184), 181- 186 (2008).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200385	50295	20 ug	$555.50		Add to Cart

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