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Home / Proteins & Peptide

PKM1 (PKM2/Variant 2), His-tag

SKU : BHP18200262

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BPS Bioscience

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Mfr.No.	40502
Description	Human pyruvate kinase muscle (PKM1), GenBank Accession No. NM_182470, full length with N-terminal His-tag, MW=59 kDa, expressed in an E. coli expression system. Specific activity: >=54 pmol/min/ug
Background	PKM1 and PKM2 are different splicing products of the PKM gene (exon 9 for PKM1 and exon 10 for PKM2). The M1 isoform (PKM1) is expressed in muscle and brain, and the M2 isoform is expressed during embryogenesis, in adipose tissue and pancreatic islets, and is the predominant form found in cancer cells.
Immunogen Region	full length
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol, and 3 mM DTT
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 50 ul reaction is conducted in abuffer containing 50 mM Tris (pH 7.4), 10mM MgCl₂, 50 mM KCl, 2 mM ADP, 10 mMphosphoenolpyruvate (PEP) at roomtemperature for 15 min. ATP production isdetected using Kinase-Glo LuminescentKinase Assay Platform. Avoid freeze/thaw cycles.
GenBank	NM_182470
Mw(kda)	59 kDa

Synonyms	Pyruvate Kinase muscle, PKM1
Uniprot	P14618

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Parkison C. et al., Biochem Biophys Res Commun. 179(1) ; 668-74 (1991). 2. Bluemlein K. et al., Oncotarget. 2011 May 22.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200262	40502	20 ug	$544.50		Add to Cart

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