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Home / Proteins & Peptide

PKLR Var2 (PKL), His-tag

SKU : BHP18200261

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Mfr.No.	40501
Description	Human pyruvate kinase variant 2 (PKL), GenBank Accession No. NM_181871, full length with N-terminal His-tag 2-543 (end), MW=59 kDa, expressed in an E. coli expression system. Specific activity: >=190,000 pmol/min/ug.
Background	PKLR is a pyruvate kinase which catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP. This reaction is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.
Immunogen Region	2-543 (end)
Tag	N-terminal His-tag
Formulation	40 mM Tris pH 8.0, 146 mM NaCl, 20% glycerol, and 3 mM DTT
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 50 ul reaction is conducted ina buffer containing 50 mM Tris (pH 7.4),10 mM MgCl₂, 50 mM KCl, 2 mM ADP, 10mM phosphoenolpyruvate (PEP) at roomtemperature for 15 min. ATP production isdetected using Kinase-Glo LuminescentKinase Assay Platform. Avoid freeze/thaw cycles.
GenBank	NM_181871
Mw(kda)	59 kDa

Synonyms	Pyruvate Kinase RBC, PKLR Var 2, PKL, PK1
Uniprot	P30613

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Bullido M.J. et al., Neurobiol. Aging. 29(10): 1160-6 (2008). 2. Russell S.T. et al., Exp. Cell. Res. 315(1) : 16-25 (2009).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200261	40501	20 ug	$544.50		Add to Cart

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