Human PKG1beta (beta) (GenBank Accession No. NM_006258), full length with N-terminal His-tag, MW = 78 kDa, expressed in Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 0.85 pmoles/min/ug
Immunogen Region
full length
Tag
N-terminal His-tag
Formulation
25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
Avoid freeze/thaw cycles.
GenBank
NM_006258
Mw(kda)
78 kDa
Synonyms
CGK1B, PRKG1B, cGMP-dependent, type I, cGKIb
Uniprot
Q13976
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Bivalacqua T.J. et al., BJU Int. 2007 Jun,99(6):1488-94. 2. Lee J.H. et al., Retrovirology. 2007 Dec 13,4:91.