cGMP-dependent Human PKG1alpha (GenBank Accession No. NM_001098512), full length with N-terminal His-tag, MW = 80 kDa, expressed in Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 579 pmoles/min/ug
Immunogen Region
full length
Tag
N-terminal His-tag
Formulation
25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
Avoid freeze/thaw cycles.
GenBank
NM_001098512
Mw(kda)
80 kDa
Synonyms
CGK1A, PRKG1A, cGMP-dependent, type I, cGKIa, PKG1alpha
Uniprot
Q13976
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Sharma,A.K.et al., J. Biol. Chem. 283 (47), 32860-32869 (2008). 2. Pohler,D.et al., FEBS Lett. 374 (3), 419-425 (1995).