JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

PKCalpha (PKCalpha), GST-tag

SKU : BHP18200179

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	40157
Description	Recombinant human PKCalpha (PKCalpha), full length, GenBank Accession No. NM_002737, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. MW= 103 kDa. Host cell line: Sf9 cells Specific activity: 3075 pmol/min/ug
Background	PKCalpha (PKC alpha) is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control(1). PKCalpha has been assigned to the chromosome region 17q22-q23.2 and has been identified as a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes (2).
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	5 mM MOPS, 2.5 mM beta-glycerophosphate, 5 mM MgCl₂, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate PKCalpha with 0.2 mg/ml CREBtide peptide substrate and 50 M (³³P)-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter. Avoid freeze/thaw cycles.
GenBank	NM_002737
Mw(kda)	103 kDa

Synonyms	AAG6, PKCA, PRKCA, PRKACA, MGC129900, MGC129901, PKCalpha protein kinase c alpha
Uniprot	P17252

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Reference	1. Coussens, L. et al. Science 233: 859-866, 1986. 2. Braz, J. C. et al. Nature Med. 10: 248-254, 2004.

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18200179	40157	10 ug	$363		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.